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5810

EFFECT OF SILICON ON SOMATIC EMBRYOGENESIS AND SHOOT REGENERATION OF DRY DATE PALM (PHOENIX DACTYLIFERA L.) CV BARTAMUDA

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Plants

Abstract

Silicon (Si) has not been observed as an essential element for plant growth, however, the beneficial effect of Si has been recognized in many plant species. The use of Si is one of the important recent trends for improving embryogenesis, growth traits and morphological characters in the field of tissue culture, for using on a commercial scale. Therefore, in this study the effect of potassium silicate (K2SiO3) and sodium silicate (Na2SiO3) at different concentrations (0.0, 0.5, 1.0, 2.0, 4.0 and 8.0 mg/l) were examined on somatic embryogenesis and regeneration percentage of dry date palm cv Bartamuda In the light of the obtained results, treatment with Si had a positive effect. The highest percentage of somatic embryogenesis formation and callus degree was obtained on Murashige and Skoog (MS) medium supplemented with 4.0 mg /l K2SiO3 from embryogenic callus derived from shoot tip explants. Moreover, this medium gave the highest number of embryos/culture and the lowest degree of browning and hyperhydricity.  However, the highest mean length of embryo was obtained on MS medium supplemented with 2.0 mg/l K2SiO3 compared with other treatments. Fresh and dry weight of embryo increased linearly with increasing concentration of Si treatments of both K2SiO3 and Na2SiO3. The highest germination percentage was obtained with embryos that matured on MS medium supplemented with 8.0 mg/l K2SiO3 or Na2SiO3. To our knowledge, this is the first study on the in vitro application of Si on dry date palm.

DOI

10.21608/ejdr.2014.5810

Keywords

in Vitro, Embryo formation, shoot tip, leaf primordial, potassium silicate, sodium silicate

Authors

First Name

Reda

Last Name

Abo El Fadl

MiddleName

E.

Affiliation

Tissue Culture Unit, Department of Genetic Resources, Ecology and Dry Land Agriculture Division, Desert Research Center, Cairo, Egypt

Email

redaaboelfadl@yahoo.com

City

-

Orcid

-

Volume

64

Article Issue

1

Related Issue

1020

Issue Date

2014-12-01

Receive Date

2018-03-29

Publish Date

2014-12-01

Page Start

65

Page End

82

Print ISSN

1687-8043

Online ISSN

2356-9875

Link

https://ejdr.journals.ekb.eg/article_5810.html

Detail API

https://ejdr.journals.ekb.eg/service?article_code=5810

Order

4

Type

Original Article

Type Code

117

Publication Type

Journal

Publication Title

Egyptian Journal of Desert Research

Publication Link

https://ejdr.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023