COTTON is known as the king of fibers and an important source of oil. The aim of this study was to evaluate the mitigating effects of three osmoprotectants, including glycine betaine (GB), proline, and chitosan each at two concentrations of 400 and 200 parts per million (ppm), 100 and 50 ppm, and 300 and 100 ppm, respectively, on water deficit stress after irrigation intervals of 10-, 15-, and 20-days, relative to the untreated (control) plants. Data indicated that prolonged irrigation interval significantly decreased growth, yield, yield components, fiber parameters, total chlorophyll, Chl. a, and Chl. b, while, proline and total soluble sugars significant increased. All drought tolerance inducers showed significant increases in cotton growth and productivity traits. Generally, GB treated plants at 400 ppm showed superior traits of all studied parameters. For example, the interaction between irrigation intervals and GB application caused significant effects on growth and productivity as well as fiber quality and chemical properties. The application of drought tolerance inducers mitigated the effects of prolonged irrigation intervals in cotton, and all drought tolerance inducers achieved higher yield and yield component values at 15 days irrigation interval compared with the control at 10 days irrigation interval. Application of 400 ppm GB could improve cotton plants endurance against the negative effect of prolonged water intervals.