ASPECTS of variations in the stem, leaf, and petiole anatomy of Crataegus monogyna Jacq., Malus domestica Borkh., Prunus armeniaca L., Prunus domestica L., Prunus avium (L.) L., Prunus persica (L.) Batsch, Prunus spinosa L., Pyrus communis L., and Rosa × damascena Herrm. (Rosaceae) have been recorded compared to fresh specimens. The present study disclosed that the anatomical characteristics of the leaf and stem are important in differentiating different species, such as leaf thickness, mesophyll, vascular bundle, and the upper and lower epidermis of the leaf. The only vascular bundle of the leaf mid vein is consistently kidney-shaped except in P. spinosa where it is circular. The petiole outline in the cross section, was between kidney and circular in all species. The data matrix was analyzed numerically using the PAST program. Species were divided into two groups based on cluster analysis and similarity percentage as species belonging to Prunus formed one group and R. damascena and P. persica were placed in another group. The current study indicates the taxonomic usefulness of the largely untapped source of anatomical attributes of Rosaceae.