THE CURRENT Study was carried out to evaluate the effect of different mineral nitrogen levels alone or combined with bio-fertilizers (Rhizobium radiobacter sp strain inoculation and Yeast) on the physical and chemical properties of soil and wheat productivity. Field experiments were carried out in sandy soil of farm at 6 October Agriculture Company, El-Qasasin, Ismailia Governorate, Egypt. Wheat grains at a rate of 60kg fed-1 were divided into to three divisions. In first division, mineral nitrogen fertilizer rates (25, 50, 75 and 100kg N fed-1) were added. In second division, grains were inoculated with Rhizobium radiobacte sp strain (PGPR) mixed with 400mL of adhesive liquid (Arabic gum). In third division, 33% N ammonium nitrate at rates (25, 50, 75 and 100kg N fed-1) were applied three times 21, 40 and 60 days after implanting and potassium sulphate (48% K2O) at a rate of 75kg fed-1 was applied on two doses after 21 and 45 days from planting. The results showed that soil pH was around 7.90 to 8.01. Soil salinity was 1.61 and 1.24 for soil treated with mineral nitrogen and a mixture of (yeast + bacteria) with a relative increasing of mean values nitrogen were 2.70, 3.63 and 5.22%. The available soil Fe, Mn, and Zn were 2.65 to 3.12mg/kg for Fe; 1.35 to 1.63mg/kg for Mn, and 0.60 to 0.85mg/kg for Zn. In conclusion, this study confirms the use of bio-fertilizers to get high-quality yields of wheat and avoid environmental pollutions.