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151892

Allele Frequency and Genetic Diversity of Some Species of Genus <i>Vicia</i> L. Using SDS-PAGE Technique

Article

Last updated: 01 Jan 2025

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Abstract

GENETIC diversity refers to genetic variability within and among species. Each individual species possesses alleles which are the source of its own unique adaptation features to the future biotic and abiotic stresses. The greater the genetic diversity within a species, the greater that this species have chances of long-term survival.
Studying seed storage proteins patterns of different species of Vicia germplasm was aimed to characterize and assess the genetic diversity and phylogenetic relationship among defined 51 accessions collected by ICARDA (International Center for Agricultural Research in Dry Area) from different countries and habitats. These represent nine Vicia species and subspecies; V. ervilia, V. monantha, V. villosa subsp. villosa, V. villosa subsp. dasycarpa, V. villosa subsp. eriocarpa, V. sativa subsp. nigra, V. sativa subsp. amphicarpa, V. sativa subsp. macrocacrpa and V. sativa subsp. sativa. Dry seeds were used to extract total storage proteins since each seed is regarded as a fixed physiological state. The pattern of variation in molecular size of total storage proteins in those accessions were studied by the numerical analysis of data obtained by using SDS-PAGE technique. The descriptive population statistics calculations were based on band presence versus absence and allele frequencies. Consequently, these data generate pertinent genetic information for the complement of passport data of ICARDA germplasm collections.

DOI

10.21608/ejbo.2021.47720.1579

Keywords

Allele frequency, Electrophoresis, Seed storage proteins, UPGMA dendrogram

Authors

First Name

Ghada E.

Last Name

El-Badan

MiddleName

-

Affiliation

Cytology and Genetics section, Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt

Email

ghadaelbadan@hotmail.com

City

Alexandria

Orcid

-

First Name

Amal W.

Last Name

Amin

MiddleName

-

Affiliation

Cytology and Genetics section, Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt

Email

amal_w_amin@yahoo.com

City

Alexandria

Orcid

-

First Name

Fatma M.

Last Name

Ashour

MiddleName

-

Affiliation

Cytology and Genetics section, Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt

Email

fatma.ashor@alexu.edu.eg

City

Alexandria

Orcid

-

First Name

Laila M.

Last Name

El-Sadek

MiddleName

-

Affiliation

Cytology and Genetics section, Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt

Email

ayyad31@hotmail.com

City

Alexandria

Orcid

-

Volume

61

Article Issue

2

Related Issue

26921

Issue Date

2021-08-01

Receive Date

2020-11-03

Publish Date

2021-08-01

Page Start

491

Page End

511

Print ISSN

0375-9237

Online ISSN

2357-0350

Link

https://ejbo.journals.ekb.eg/article_151892.html

Detail API

https://ejbo.journals.ekb.eg/service?article_code=151892

Order

78

Type

Original Article

Type Code

111

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

MainTitle

Allele Frequency and Genetic Diversity of Some Species of Genus <i>Vicia</i> L. Using SDS-PAGE Technique

Details

Type

Article

Created At

22 Jan 2023