THE TRADITIONAL methods for hyaluronic acid (HA) production are costly and require extensive purification of the basic product. These techniques are based on animal extractions and the fermentation from micro-organisms. This research aims to develop a new, improved method for the stable HA production by irradiation Streptococcus equi (S. equi) to low-power UV light under the ethyl methanesulfonate (EMS) influence as a chemical mutagenic agent as well as certain amino acids (L-glutamic acid, L-aspartic acid, L-arginine, L-glycine, and L-lysine) for biosynthesizing metal nanoparticles. The results clearly show the steady development of the control HA (0.045g/L) to 0.25, and 0.27g/L during UV light (254nm) exposure for four minutes and treatments of 25μg/mL (EMS) and amino acids (glutamic acid and lysine). Under these conditions, the incubation of S. equi resulted in an increase in the HA yield by factors of 5.55 and 6, respectively. The molecular mass and chemical analysis of the obtained HA showed considerable similarities compared to the standard sample. The obtained HA was utilized as a capping agent for the biosynthesis of silver (13.0±2.9nm), gold (21.8±1.9nm), and ultra-small zero-valent iron (4.1±0.3nm) nanoparticles. This method provided ample achievement and is effective in HA production.