PATHOGENIC fungi are among the most serious biotic agents that attack plants and cause considerable losses in crop yield. Therefore, a sincere effort is being made to provide safe and inexpensive ecofriendly innovative approaches for managing plant diseases and reducing the fungicide pressure in our agro-ecosystem. In this study, Bradyrhizobium japonicum Asw1 was successfully used for suppressing the root rot disease of fenugreek (Trigonella foenum-graecum L.) caused by Fusarium solani. Strain Asw1 exhibited significant antifungal activity revealing remarkable antagonistic effect against the pathogen. The results showed that T. foenum-graecum was susceptible to fungal infection which severely affected the growth and metabolite content of the plant. Treatment with strain Asw1 resulted in a sharp reduction of disease incidence from 80 to 13%. Growth attributes, e.g., plant height (cm), number of leaves per plant, fresh and dry weight (g) of treated plants were significantly enhanced. The contents of chlorophylls, proteins, carbohydrates, flavonoids, phenolics and tannins were significantly (P< 0.05) reduced in the infected plants. In the treated plants, protein and carbohydrate contents were not enhanced compared to control. However, the content of total flavonoids was significantly accumulated in the treated plants which might trigger their resistance against fungal infection.