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31032

Changes in the Profiling of Fatty Acids of <i> Glycine max </i> L. (Soybean) Callus after Mutagen Treatments

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Last updated: 22 Jan 2023

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Abstract

THE AIM of present study was to produce healthy friable calli from soybean cultivar Giza 111 and studying the effect of sodium azide (SA) and ethidium bromide (EtBr) mutagens on its fatty acids (FAs) profile. Seeds were surface sterilized with clorox and ethanol, then cultured on solid Murashige and Skoog medium (MS) to produce 14-days-old in vitro plantlets. Callus induction from hypocotyl (H), cotyledon (C), epicotyl (E) and leaf (L) explants were tested using different combinations of 2,4-dichlorophenoxy acetic acids (2,4-D) and N6-benzyladenine (BA). M4 (MS+ 0.5mg/L BA) medium was found to be the supreme medium for callus proliferation. Both H-and E-explants were the best explants for this proliferation. Growth dynamics of H- and E-calli was established. The best harvesting time for both calli was the 4th week. H-calli were the most rapidly growing one. The concentrations 0.5, 1.0 and 1.5mM of each mutagen with two soaking times (½ and 1hr) were used for the treatment of H-calli. After mutagenic treatment, treated-H-calli were cultured on callus induction media. A month later, FAs profiling of mutated and control H-calli were performed using gas liquid chromatography (GLC). The Fw pattern of SA and EtBr-treated-H-calli were similar to the control one but with lower values. Saturated (SFAs) and unsaturated-fatty acids (UFAs) contents were raised up by increasing the concentrations of SA and time of incubation. In EtBr-treated-H-calli, marked elevations of SFAs over UFAs were recorded exhibiting a switch in their balance by increasing concentrations and soaking times.

DOI

10.21608/ejbo.2019.6176.1250

Keywords

Calli, 2, 4-dichlorophenoxyacetic acids, fatty acids, <i> Glycine max </i> L, N6-benzyladenine mutagens

Authors

First Name

Rehab

Last Name

Hafez

MiddleName

M.

Affiliation

Botany and Microbiology Department, Faculty of Science, Cairo University, Giza, Egypt

Email

rehabhafez@sci.cu.edu.eg

City

Giza

Orcid

0000-0003-0528-288X

First Name

Abdallah

Last Name

Mohammed

MiddleName

A.Y.

Affiliation

Biotechnology/Bimolecular Chemistry Program, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt

Email

20140459@std.sci.cu.edu.eg

City

Giza

Orcid

-

First Name

Abd El-Rahman

Last Name

Abd El-Naby

MiddleName

M.

Affiliation

Biotechnology/Bimolecular Chemistry Program, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt

Email

bodyclan1996@yahoo.com

City

Giza

Orcid

-

First Name

Ali

Last Name

Tolba

MiddleName

-

Affiliation

Biotechnology/Bimolecular Chemistry Program, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt

Email

ali_ali20111143@yahoo.com

City

Giza

Orcid

-

First Name

Eslam

Last Name

Khalifa

MiddleName

Y.M.

Affiliation

Biotechnology/Bimolecular Chemistry Program, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt

Email

eslamyousif36@yahoo.com

City

Giza

Orcid

-

First Name

Hamed

Last Name

Hamed

MiddleName

M.

Affiliation

Biotechnology/Bimolecular Chemistry Program, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt

Email

playhard-workhard@hotmail.com

City

Giza

Orcid

-

First Name

Mohammed

Last Name

Abdullah

MiddleName

M.K.

Affiliation

Biotechnology/Bimolecular Chemistry Program, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt

Email

modda996@gmail.com

City

Giza

Orcid

-

First Name

Mohammed

Last Name

Ahmed

MiddleName

M.F.

Affiliation

Biotechnology/Bimolecular Chemistry Program, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt

Email

mmfa9600@hotmail.com

City

Giza

Orcid

-

First Name

Mohammed

Last Name

Hekal

MiddleName

S.

Affiliation

Biotechnology/Bimolecular Chemistry Program, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt

Email

moh4sh96@gmail.com

City

Giza

Orcid

-

First Name

Dalia

Last Name

Ali

MiddleName

H.A.

Affiliation

Biotechnology/Bimolecular Chemistry Program, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt

Email

memodoshe4@gmail.com

City

Giza

Orcid

-

Volume

59

Article Issue

3

Related Issue

9474

Issue Date

2019-11-01

Receive Date

2018-11-22

Publish Date

2019-11-01

Page Start

679

Page End

694

Print ISSN

0375-9237

Online ISSN

2357-0350

Article File

EJBO_Volume 59_Issue 3_Pages 679-694.pdf

PDF . 2.3MB

Link

https://ejbo.journals.ekb.eg/article_31032.html

Detail API

https://ejbo.journals.ekb.eg/service?article_code=31032

Order

11

Type

Original Article

Type Code

111

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023