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30547

Identification of Different Isolated Fungi from Scaly Debris of <i>Colocasia</i> Corm With Potential Production of Cellulase

Article

Last updated: 01 Jan 2025

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Abstract

THE AIM of the present work is to isolate and identify the different fungi from the debris of the brown outer scaly leaves of Colocasia corm. The isolated fungi were tested for cellulase producing activity. The species were primarily recognized on morphological bases and then identified on molecular level. The Internal Transcribed Spacer (ITS) domain of the ribosome large subunit (LSU)-comprising the ITS1/5.8S/ITS2 regions (≥ 500bp) was subjected to Sanger sequencing for the new isolates using primer pair ITS1/ITS4. BLAST analysis indicated entities of the eight-isolated fungal isolates. Among the isolates, three of the species were found-closely related to Fusarium solani whereas, the other five isolations were found to be closely related to F. proliferatum, F. fujikuroi, F. equiseti, Penicillium lanosum and P. flavigenum. Screening of the fungal species for cellulase production"was performed on cellulose-agar plates using clear zone technique. The results showed that out of theses isolates, the maximum zone of clearance was obtained for isolate that is closely related to P. lanosum, whereas, the minimum-zone of clearance was recorded for isolates closely related to Fusarium solani. Penicillium isolate showing significant-potentiality in cellulase production will be a subject for a detailed investigation.

DOI

10.21608/ejbo.2019.7575.1283

Keywords

Plant debris, <i> Penicillium, Fusarium </i>, rDNA, ITS, Cellulase

Authors

First Name

Nahla

Last Name

Bouqellah

MiddleName

Alsayed

Affiliation

Taibah University, Faculty of Science, Biology Department, P.O. Box 25233, Almadinah Al-Munawara, Saudi Arabia

Email

nahla.b@hotmail.co.uk

City

Almadinah Al-Munawara

Orcid

-

Volume

59

Article Issue

2

Related Issue

6794

Issue Date

2019-08-01

Receive Date

2019-01-31

Publish Date

2019-08-01

Page Start

513

Page End

522

Print ISSN

0375-9237

Online ISSN

2357-0350

Link

https://ejbo.journals.ekb.eg/article_30547.html

Detail API

https://ejbo.journals.ekb.eg/service?article_code=30547

Order

18

Type

Original Article

Type Code

111

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

MainTitle

Identification of Different Isolated Fungi from Scaly Debris of <i>Colocasia</i> Corm With Potential Production of Cellulase

Details

Type

Article

Created At

22 Jan 2023