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31447

Evaluation of Bacteria from Soil and Rhizosphere as Herbicidal Candidates of Some Broadleaf Weeds

Article

Last updated: 01 Jan 2025

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Abstract

The use of bacteria in biological weed control is an alternative ecofriendly way to tackle weed problems and reduce the risk of herbicide resistance. In this study, we obtained four main active bacterial isolates under the genera Pseudomonas, Bacillus and Xanthomonas from Wadi El Natroun region. All the tested bacterial isolates caused high significant reductions
in seed germination and seedling growth of Convolvulus arvensis and Portulaca oleracea. Bioassaying ethyl acetate crude extracts of these isolates showed that Pseudomonas sp. (isolate 1) was the most active against seedling stage of Portulaca oleracea. The cultural filtrate of the same isolate caused 100% reduction when assayed on seed germination, shoot and root length of Convolvulus arvensis and Portulaca oleracea. At the highest concentration (40mg/ml) of the crude ethyl acetate extract, the reduction percentage in total biomass fresh weights of Portulaca oleracea and Convolvulus arvensis seedlings reached 71.27 and 39.37%, respectively. The EC50 values (concentration that inhibited growth by 50%) were 1.3 and 1.64mg/ml for Portulaca oleracea and Convolvulus arvensis, respectively. Comparative 16S rRNA gene sequencing analysis and biochemical characterization demonstrated that isolate FS15 was member of the genus Pseudomonas and belongs to the Pseudomonas aeruginosa group with Pseudomonas aeruginosa strain NR 113599.1 as the closest relative (99.5% sequence similarity). In conclusion, Pseudomonas aeruginosa has high potential to be developed as natural bacterial herbicide and may be used in broadleaf weed control.

DOI

10.21608/ejbo.2018.4172.1186

Keywords

rhizobacteria, Biocontrol agent, <i> Pseudomonas aeruginosa </i>, Noxious weeds, Bioherbicides

Authors

First Name

Merhan

Last Name

Tawfik

MiddleName

M.

Affiliation

Plant Protection Department, Desert Research Center, El-Matariya, Cairo, Egypt

Email

merhangalal@yahoo.com

City

obour city

Orcid

-

First Name

Nevin

Last Name

Ibrahim

MiddleName

A.

Affiliation

2Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

nevin_a_ibrahim@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Balah

MiddleName

A.

Affiliation

Plant Protection Department, Desert Research Center, El-Matariya, Cairo, Egypt

Email

mbaziz1974@gmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Abouzeid

MiddleName

M.

Affiliation

Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

m_abouzaid@sci.asu.edu.eg

City

-

Orcid

-

Volume

59

Article Issue

2

Related Issue

6794

Issue Date

2019-08-01

Receive Date

2018-08-05

Publish Date

2019-08-01

Page Start

283

Page End

291

Print ISSN

0375-9237

Online ISSN

2357-0350

Link

https://ejbo.journals.ekb.eg/article_31447.html

Detail API

https://ejbo.journals.ekb.eg/service?article_code=31447

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Type

Original Article

Type Code

111

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

MainTitle

Evaluation of Bacteria from Soil and Rhizosphere as Herbicidal Candidates of Some Broadleaf Weeds

Details

Type

Article

Created At

22 Jan 2023