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Molecular characterization and biocontrol-traits evaluation of <i> Trichoderma virens </i> TVH3 against Fusarium oxysporum, the causal agent of tomato wilt.

Article

Last updated: 22 Jan 2023

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Abstract

In atte mpt to screen for new biological control agents (BCA) with high activity against soil-borne fungal pathogens, an isolate of the BCA Trichoderma virens was obtained, identified using molecular techniques and named TVH3. The antagonistic activities of
this isolate were tested against Fusarium oxysporum, a major soil-borne fungal pathogen of diverse crops. Our results revealed that T. virens TVH3 displayed high antagonistic potential against F. oxysporum via different mechanisms to control its prey, such
mechanisms include competition for the available space and nutrients, mycoparasitism and
production of antimicrobial secondary metabolites including volatile organic compounds
(VOCs). The antifungal soluble metabolites in Trichoderma's culture filtrate were found to
be thermal-stable and T. virens TVH3 was found to possess high natural immunity against
antimicrobial metabolites in F. oxysporum culture filtrate. In the biocontrol assay, isolate
T. virens TVH3 was applied as seed coat on tomato seeds before planting them in heavily
infested soil with pathogenic strain of F. oxysporum and the obtained results revealed that such seed treatment had significantly reduced wilt signs on plants and provided the seedlings with effective shield against pathogenic infection.

DOI

10.21608/ejbo.2018.964.1079

Keywords

Biocontrol, <i> Trichoderma </i>, <i> Fusarium </i>, Biofungicide, Tomato, Wilt

Authors

First Name

Alsayed

Last Name

Alfiky

MiddleName

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Affiliation

Genetics Department, Faculty of Agriculture, Tanta University, Tanta, Egypt

Email

alfiky@agr.tanta.edu.eg

City

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Orcid

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First Name

Medhat

Last Name

Eldenary

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Tanta University, Tanta, Egypt

Email

medhat91@yahoo.com

City

-

Orcid

-

Volume

59

Article Issue

1

Related Issue

5166

Issue Date

2019-04-01

Receive Date

2017-04-18

Publish Date

2019-04-01

Page Start

68

Page End

80

Print ISSN

0375-9237

Online ISSN

2357-0350

Link

https://ejbo.journals.ekb.eg/article_16427.html

Detail API

https://ejbo.journals.ekb.eg/service?article_code=16427

Order

7

Type

Original Article

Type Code

111

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

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Article

Created At

22 Jan 2023