MULTI walled carbon nanotubes (MWCNT) are gaining more attention to be used in industry and agriculture. The effect of this new nanomaterial should be investigated in order to gain more understanding about its effect on living organisms. In this study, pennycress plant was used as a model to determine the effect of MWCNT on the whole plant as well as its free genomic DNA. Germinated seedlings of pennycress plants were grown on MS agar media supplemented with 25,100 and 200μg/μl MWCNT for 6 days. The root elongation assay indicated that 25μg/μl of MWCNT enhanced the plant growth while the growth rate was reduced at higher concentrations. The gel shift binding assay indicated that MWCNT exhibited the ability to bind the genomic DNA at higher concentrations of 100 and 200μg/μl while no binding was observed at lower concentration of 25μg/μl. This study also showed that the plant pigment was highly reduced at higher concentrations as will be further discussed.