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13989

Effect of Sodium Nitro-Prusside (SNP) Preatreatment on Ammonia Assimilating Enzymes of Salt Stressed Tomato Leaves (<i> Lycopersicon esculentum </i>)

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Last updated: 01 Jan 2025

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Abstract

THE EFFECTS of treatment with 100mM NaCl on photosynthetic activity, protein and proline contents , activities of key nitrogen metabolism enzymes, nitrate reductase (NR) and nitrite reductase (NiR), ammonia assimilating enzymes: Glutamine synthetase (GS), glutamate synthase (GOGAT) and glutamate dehydrogenase (NADH-GDH) activities and finally ammonia and nitrate contents were investigated in 40-day old leaves of tomato plants. There was a decline in total protein (TP) and insoluble protein (InsP) fractions accompanied with a significant increase in proline and soluble protein contents in response to NaCl – stress. Under salinized conditions, there was a significant inhibition in all tested leaf gas exchange parameters, stomatal conductance (gs), internal CO2 concentration (Ci) and hence, CO2 assimilation (A). Also, a significant inhibition of NR & NiR enzymes and a strongly decrease in nitrate content were observed. In contrast, ammonia assimilating enzymes (GS, GOGAT, NADH-GDH and NAD-GDH) activities were obviously increased in NaCl – salinized tomato leaves, this accompanied with a significant increase in ammonia content. Soaking tomato seeds in 10μM sodium nitroprusside (SNP) for 8h were elevated-to some extent- all the studied parameters and there was an improvement in TP, proline, all gas exchange parameters, NR & NiR activities and nitrate content. While ammonia assimilating enzymes (GS, GOGAT, NADH-GDH and NAD-GDH) activities and ammonia content were significantly decreased compared to NaCl - salinized tomato leaves.

DOI

10.21608/ejbo.2018.3064.1160

Keywords

salt stress, Sodium nitroprosside (SNP), Nitrogen metabolism enzymes, Ammonia assimilating enzymes, tomato plants

Authors

First Name

Hala

Last Name

Aly

MiddleName

Ezzat Mouhamed

Affiliation

Botany Department, Faculty of Science, Alexandria University, Alexandria, Egypt

Email

halaamzn99@yahoo.com

City

alexandria

Orcid

-

First Name

Nabil

Last Name

Saber

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Alexandria University, Alexandria, Egypt

Email

nabilsaber@hotmail.com

City

-

Orcid

-

First Name

Amany

Last Name

Mohamed

MiddleName

Gaber

Affiliation

Biological and Geological Department, Faculty of Education, Alexandria University, Alexandria, Egypt

Email

-

City

-

Orcid

-

Volume

58

Article Issue

3

Related Issue

4032

Issue Date

2018-12-01

Receive Date

2018-03-01

Publish Date

2018-12-01

Page Start

491

Page End

500

Print ISSN

0375-9237

Online ISSN

2357-0350

Link

https://ejbo.journals.ekb.eg/article_13989.html

Detail API

https://ejbo.journals.ekb.eg/service?article_code=13989

Order

15

Type

Original Article

Type Code

111

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

MainTitle

Effect of Sodium Nitro-Prusside (SNP) Preatreatment on Ammonia Assimilating Enzymes of Salt Stressed Tomato Leaves (<i> Lycopersicon esculentum </i>)

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Article

Created At

22 Jan 2023