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The Effect of Seed Presoaking with KNO3 on Seed Germination, Proline, Protein Pattern, ß-Amylase and Mineral Composition of Two Faba Bean Cultivars Treated with NaCl

Article

Last updated: 01 Jan 2025

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Abstract

SEEDS of two broad bean cultivars (Vicia faba, cv. Nobaria 3 and cv. Sakha 3) were obtained from Mallawi Agriculture Research Center, El Minia Governorate, Egypt. Different salinization levels (0, 40, 80, 120 and 160mM NaCl) were used and both cultivars were presoaked in either water (Reference control) or potassium nitrate (3mM), then left for germination for five days. Plants transported to the field and further growth for 65 days from sowing. The final germination percentage the two broad bean cultivars of Vicia faba c were decreased by increasing salinity in the rooting medium. Seed presoaking in KNO3 resulted in remarkable increase in final germination percentage especially in cultivar Sakha 3. Both cultivars accumulated proline in both different organs reached 3 folds higher than absolute control, Seed presoaking in KNO3 had a significant increase in proline contents especially in shoots regardless the salinity levels used. Mineral composition the plants of the two broad bean cultivars showed various responses with increasing salinity. Seed presoaking in KNO3 resulted in remarkable decrease in Na and increased K contents in tested plants. One band of β-amylase were detected under different concentrations of NaCl combined with KNO3. The intensity of the bands varied between various treatments. Exposure of Sakha 3 and Nobaria 3 cultivars to different concentrations of NaCl salinity and those presoaked in KNO3 (3mM) were produced marked changes in their protein pattern.

DOI

10.21608/ejbo.2018.3423.1166

Keywords

proline, Protein pattern, Faba bean cultivars, β -amylase, Mineral compositions

Authors

First Name

Gaber

Last Name

Abdel-Baki

MiddleName

K.

Affiliation

Botany and Microbiology Department, Faculty of Science, Minia University, El-Minia, Egypt

Email

gaberahmed137@yahoo.com

City

-

Orcid

-

First Name

M.

Last Name

Shaddad

MiddleName

A.K.

Affiliation

otany Department, Faculty of Science, Assiut University, Assiut, Egypt

Email

-

City

-

Orcid

-

First Name

Doaa

Last Name

Mostafa

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Minia University, El-Minia, Egypt

Email

moustafadoaa@yahoo.com

City

El-Minia

Orcid

-

First Name

Al-Shimaa

Last Name

Rafat

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Minia University, El-Minia, Egypt

Email

-

City

-

Orcid

-

Volume

58

Article Issue

3

Related Issue

4032

Issue Date

2018-12-01

Receive Date

2018-04-04

Publish Date

2018-12-01

Page Start

445

Page End

461

Print ISSN

0375-9237

Online ISSN

2357-0350

Link

https://ejbo.journals.ekb.eg/article_7903.html

Detail API

https://ejbo.journals.ekb.eg/service?article_code=7903

Order

12

Type

Original Article

Type Code

111

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

MainTitle

The Effect of Seed Presoaking with KNO3 on Seed Germination, Proline, Protein Pattern, ß-Amylase and Mineral Composition of Two Faba Bean Cultivars Treated with NaCl

Details

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Article

Created At

22 Jan 2023