LENTIL (Lens culinaris Medikus) is an important seed legume crop cultivated worldwide as human food. Little is known about the molecular structure of the Egyptian lentil. Inter-varietal variation and cultivar identification are crucial for breeding and gene bank conservation of this plant worldwide. The SDS–PAGE and SRAP-PCR techniques were used to detect some biochemical and molecular markers of six Egyptian lentil genotypes (Sinai 1, Line 9, Giza 1, Giza 4, Giza 51 and Giza 370). The genetic distance between cultivars was also estimated from banding patterns. The results of SDS-PAGE revealed a total of 21 bands with molecular weight ranging from about to 6-102 KD. The maximum number of bands (14) appeared in Giza 4, while the minimum number of bands (10) appeared in Giza 1. Electrophoresis analysis among six lentil cultivars showed 80.95% polymorphism, while DNA variations were explored using ten combinations of SRAP markers. SRAP analysis among six lentil cultivars showed 61.54% polymorphism. The highest similarity index (0.869) was found between Giza 51and Giza 370. The lowest similarity index (0.603) was found between Giza 1 and Giza 4. The markers used in this study created polymorphic bands among the different cultivars that can be utilized as molecular markers for their differentiation. The obtained data indicated that SRAP and SDS-PAGE systems can efficiently identify and differentiate between the selected cultivars. Also these results, enhancing the available knowledge of lentil genetic resources in Egypt, may contribute to their conservation and utilization in breeding programs.