THE AIM of this work was to evaluate the impact of sources of waste water pollution on Ismailia canal and irrigated plants. The industrial sources along Ismailia canal produce large wastes that disturb the environmental balance. This study was based on investigating the growth and biochemical characteristics of Pisum sativum seedlings irrigated with industrial waste water collected from three selected stations of Ismailia canal. Results revealed an enrichment of organic matter and heavy metals in polluted water. Irrigation of plants using waste water affected germination, physiological, biochemical and growth characteristics of Pisum sativum. Irrigation with polluted water reduced photosynthetic pigments, total nitrogen and proteins in plant. Uptake and translocation of heavy metals and metal ions were higher in the polluted Pisum sativum which produced more metabolites to combat metal toxicity. High activity of antioxidant enzymes (Catalase, peroxidase and polyphenol oxidase), the contents of soluble sugars, anthocyanine, proline and new protein bands were induced.