CHILLING stress induced a significant increase of electrolyte leakage (E.L.%) of broad bean leaves (Vicia faba cv. Nobaria, 1) and that was associated with a significant decrease of normalized concentrations of K, Ca, Mg, P, Fe ions and N (as NO-3) and increase of Cu and Zn in leaves and roots. There was a significant accumulation of H2O2 and malondialdehyde (MDA) contents accompanied with a significant increase of enzymatic antioxidants (SOD, CAT, APx, GR and PPO) activities. During the 6 h reacclimation period, the CAT, APx, GR and PPO activities were significantly increased compared to those of chill-stressed plants, whereas SOD significantly decreased. There are several polypeptides bands were markedly appeared in leaves and roots during the exposure to low temperature and 6 h reacclimation period. The results revealed that chilling temperature had an inhibitory effect on the plasma membrane integrity due to enhancement of ROS generation and that might associate with an increase of enzymatic antioxidants activities and appearance of several stress proteins during chilling and reacclimation periods.