S UCCESSIVE leaf extracts of three Egyptian plant species: Datura stramonium L., Withania somnifera (L.) Dun. in DC. and Ziziphus spina-christi (L.)Willd were studied for their antimicrobial activity against susceptible and Multi-Drug Resistant (MDR) bacteria isolated from chickens. Four bacterial spp. namely: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella sp. were isolated from 463 different chicken samples. The sensitivity of these bacterial isolates was tested against 11 types of antibiotics: Ampicillin, Amoxicillin, Cefaclor, Gentamicin, Amikacin, Tobramycin, Ciprofloxacin, Nalidixic acid, Erythromycin, Tetracycline and Vancomycin. The isolates of E. coli, S. aureus, P.aureugenosa and Salmonella sp. showed notable bacterial resistance against the used antibiotics with percentages: (68.18%), (59.09%), (54.54%) and (54.54%); respectively. Chloroform extract of Datura stramonium showed the highest antibacterial activity against all the studied bacterial isolates with MICs values varying from 75.0 to 0.037 mg/ml. In conclusion, the present work indicated the potentiality of Datura stramonium chloroform extract as a promising national source for drug industry to produce antibiotics to be used against pathogenic multidrug resistant bacteria.