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Production of Extracellular Anti-leukemic Enzyme L-asparaginase from Fusarium solani AUMC 8615 Grown under Solid-State Fermentation conditions: Purification and Characterization o

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Last updated: 01 Jan 2025

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Abstract

POTENTIALITIES of twenty four fungal isolates were investigated for their ability to produce L-asparaginase using modified Czapek's Dox medium. Fusarium solani AUMC 8615 was selected as the most potent fungal strain for the enzyme production under solid state fermentation (SSF) using agro-industrial residues. Wheat bran supported maximum enzyme production followed by rice bran and corn cob. The optimum conditions for maximum production of L-asparaginase under SSF occurred on the fifth day of incubation at 30°C, pH 8.0, 60% of initial moisture content and supplementation with 0.2% (w/v) glucose and 0.5% (w/v) ammonium sulphate. L-asparaginase was purified to homogeneity by ammonium sulphate precipitation, gel filtration and ion exchange chromatography giving 299.58 purification fold. SDS-PAGE showed that the purified enzyme consists of two subunits of molecular weights 70 and 80 kDa, respectively. The enzyme was efficiently immobilized by covalent binding with activated charcoal giving an immobilization yield of 80.40%. Optimum pH values were 9.0 and 8.0 for free and immobilized enzyme, respectively. While, Optimum reaction temperatures were 37°C and 45°C for free and immobilized enzyme, respectively. After incubation for 2 hr at 37°C, the relative activity of free enzyme decreases to 35% whereas for the immobilized enzyme decreased only to 57% at 45°C.

DOI

10.21608/ejbo.2016.3776

Keywords

L-asparaginase, solid-state fermentation, Fusarium solani, Purification, Immobilization

Volume

56

Article Issue

3

Related Issue

507

Issue Date

2016-09-01

Receive Date

2016-05-17

Publish Date

2016-09-30

Page Start

799

Page End

816

Print ISSN

0375-9237

Online ISSN

2357-0350

Article File

EJBO_Volume 56_Issue 3_Pages 799-816.pdf

PDF . 634.0kB

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https://ejbo.journals.ekb.eg/article_3776.html

Detail API

https://ejbo.journals.ekb.eg/service?article_code=3776

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16

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Original Article

Type Code

111

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

MainTitle

Production of Extracellular Anti-leukemic Enzyme L-asparaginase from Fusarium solani AUMC 8615 Grown under Solid-State Fermentation conditions: Purification and Characterization of The Free and Immobilized Enzyme

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Article

Created At

22 Jan 2023