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Purification and Characterization of New Alkaline L-methioninase from Aspergillus ustus AUMC 1051 Grown under Solid-State Fermentation Conditions.

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Last updated: 01 Jan 2025

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Abstract

 ALKALINE L-methioninase (E.C.4.4.1.11) from Aspergillus ……. ustus AUMC 1051 was obtained in a good yield amounting to 1321 Uml-1 (99.56 Ug-1 bran) under solid state fermentation (SSF) of wheat bran. The enzyme was purified 15.83-fold with 62.63% yield after three steps of purification involved ammonium sulfate precipitation, Sephadex G-100 gel filtration and DEAE-cellulose ion exchange chromatography. The purified enzyme had a molecular mass of 46 kDa under denaturating conditions and an isoelectric point of 6. Maximal activity was recorded at pH 8.5 and 35oC. Good stability of the purified enzyme was detected over wide pH values ranging from 8 to 10 and temperature up to 50oC. The enzyme retained its full activity after 6 days of storage at 4oC. Four weeks was found the T1/2 of its activity. Vmax and Km, of the purified enzyme were found to be 820 Uml-1 and 1.6 mM, respectively. Alkaline L-methioninase activity was stimulated by Na+ and Co+2 and strongly inhibited by Hydroxylamine, iodoacetate, Hg+2 and Cu+2. The enzyme was proved to be glycoprotein containing -SH group in its catalytic site.

DOI

10.21608/ejbo.2016.3775

Keywords

solid-state fermentation, Alkaline L-methioninase, Purification, Characterization, Aspergillus ustus

Volume

56

Article Issue

3

Related Issue

507

Issue Date

2016-09-01

Receive Date

2016-05-30

Publish Date

2016-09-30

Page Start

785

Page End

798

Print ISSN

0375-9237

Online ISSN

2357-0350

Article File

EJBO_Volume 56_Issue 3_Pages 785-798.pdf

PDF . 360.1kB

Link

https://ejbo.journals.ekb.eg/article_3775.html

Detail API

https://ejbo.journals.ekb.eg/service?article_code=3775

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15

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Original Article

Type Code

111

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

MainTitle

Purification and Characterization of New Alkaline L-methioninase from Aspergillus ustus AUMC 1051 Grown under Solid-State Fermentation Conditions.

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Article

Created At

22 Jan 2023