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Characterization of a Novel Specific Mouse Monoclonal Antibody Targeted to Envelope Protein E1/E2 of Hepatitis C Virus

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Last updated: 01 Jan 2025

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Abstract

 HEPATITIS C virus has been proven to be a major disease over …….. the world and widely present in Egypt. The aim of this study was to propagate, characterize and test the reactivity of the hybridoma cell line (7G9) which produces a novel mouse monoclonal antibody (MoAb) targeting HCV E1/E2 envelope protein. The propagation of this hybridoma cell line (7G9) was generated using standard hybridoma technology. Isotyping of the generated mouse antibody was done by commercial ELISA kit. The reactivity of (7G9) was assessed by ELISA plates coated by HCV E1/E2 derived from cell lysate transfected by plasmid expressed HCV E1/E2. The generated monoclonal antibody (7G9) was found to be an IgM antibody with a kappa light chain. ELISA showed that (7G9) reacted with cell lysate expressed HCV E1/E2 and (7G9) presented no cross-reactivity with different antigens such as Brucella abortus, Salmonella typhi and Hepatitis B Virus (HBV) antigens. ELISA revealed high reactivity of (7G9) with HCV E1 (a.a. 315-323) antigen. Thus, the mouse monoclonal antibody (7G9) can be used for immunodiagnosis of HCV infection by detection of HCV E1/E2 antigens.

DOI

10.21608/ejbo.2016.2734

Keywords

Hepatitis C virus, Envelope Protein E1/E2, Hybridoma cell line (7G9)

Volume

56

Article Issue

3

Related Issue

507

Issue Date

2016-09-01

Receive Date

2016-02-07

Publish Date

2016-09-30

Page Start

669

Page End

677

Print ISSN

0375-9237

Online ISSN

2357-0350

Article File

EJBO_Volume 56_Issue 3_Pages 669-677.pdf

PDF . 387.6kB

Link

https://ejbo.journals.ekb.eg/article_2734.html

Detail API

https://ejbo.journals.ekb.eg/service?article_code=2734

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13

Type

Original Article

Type Code

111

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

MainTitle

Characterization of a Novel Specific Mouse Monoclonal Antibody Targeted to Envelope Protein E1/E2 of Hepatitis C Virus

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Article

Created At

22 Jan 2023