THIS STUDY aimed to control two selective fungi (Aspergillus parasiticus and Penicillium commune, causing biodeterioration of books and documents in storage areas of libraries) using antifungal drugs, essential oils (added singly and in combination), gamma irradiation and laser radiation. The results obtained indicated that fluconazole and clotrimazole were effective against A. parasiticus and P. commune, while nystatin was of lesser effect .P. commune showed more resistance than A. parasiticus to the antifungals (MIC60 determination). Cinnamon and clove oils were efficient antifungal agents, while garlic and thyme oils have no reasonable activities. The combination treatment revealed some synergestic interaction between garlic and thyme oils with antifungal drugs. Generally the highest control of both fungal species was shown by single treatment of clotrimazole. It was found that the two fungi responded differently against γ- irradiation .A. parasiticus was more tolerant than P .commune in dry biomass, while they were equal in γ- irradiation tolerance in the cfu / plate. The D10 determination revealed more tolerance of P. commune than A. parasiticus. The two fungal species showed resistance in their growth parameters (cfu and dry biomass) with laser beam treatment for 1-5 min. Reductions of 75% to 95% of control was detected after 10 and 15 min exposure to laser beam. The lethal effect, however, was observed after 20 min for both fungi.