THE PRESENT study was carried out in El-Khmseen canal, which …. receives irrigation water from a main, AL-Zomar canal, which passes, along Saft El-Laban town in Giza Governorate. El-Khamseen canal lies about 8.2 km southwest of Cairo University, Giza, Egypt. The canal is contaminated with the agricultural effluents, in addition to a lot of domestic pollutants and discharges resulting from the small industrial units such as paints and ceramic manufacturing. Additional sources of pollution are burning of charcoal and garbage by the farmers and the traffic emissions. The study was focused on the assessment of heavy metal content in two aquatic and one canal bank macrophytes in comparison with water, sediment and soil. Different plant parts (viz. rhizomes, living leaves, dead leaves and living roots) representing two aquatic macrophytes (Cyperus alopecuroides and Phragmites australis) and a third canal bank species; Phragmites australis. Sediments, soil, and water, were analyzed for Mn, Cu, Zn, Cd, Pb and Fe. Results elucidated that concentrations of Mn, Cu and Pb were higher in soils than in sediments. Roots were the most efficient hyperaccumulating organs, giving higher bioconcentration factor (BCF) values. The contents of heavy metals in the studied species followed the order: Fe > Mn > Zn > Cu > Pb > Cd. The investigation elucidated that the living roots of the studied species are hyperaccumulators for Fe, moderate accumulators for Mn, and Zn, poor accumulators for Cu and Pb and scarce accumulators for Cd. The content of all metal ions in the different organs of the studied species obeyed the order: living roots > dead roots > dead leaves > living leaves > culms and rhizomes. High Translocation Factor (TF) values of the test species confirm the capability of their roots to accumulate heavy metals and their translocation to the shoots. Therefore, rhizofiltration was found to be the best mechanism to explain that Phragmites australis and Cyperus alopecuroides are promising species for phytoremediation of wastewater and soil.