EVALUATION of genetic diversity among 10 species of Brassicaceae have been performed based on variations in seed protein electrophoretic patterns as revealed by SDS-PAGE and ISSR analyses, separately and in combination. The maximum genetic similarity was observed between Brassica nigra and B. rapa, while the lowest genetic similarity was observed between B. nigra and Sisymbrium irrio. Cluster analysis generated a dendrogram that separated the studied taxa in two main clusters; the five species of tribe Brassiceae were grouped close to each other in one cluster with Sisymbrium irrio. The remaining studied species; Capsella bursapastoris, Erysimum cheiranthoides, Mathiola longipetale and Thlaspi arvense, were grouped in a second cluster.