TE AIM of this study was to assess the effectiveness of mixed Bacillus strains applications. These strains are B. megaterium var. phosphaticum which efficient in dissolving phosphate, B. circulansas potassium release bacteria and B. polymyxa as nitrogen fixer. The effect of the applied mixed bacillus strains, natural fertilizers and their interactions on the growth, nutrients uptake of nitrogen, phosphorus and potassium, and yield parameters of wheat plants grown in a potted soil under natural conditions were studies. The results revealed that, the application of biofertilizers (Bacillus strains) with natural fertilizers (rock phosphate and feldspar) in alkaline soil caused a significant increase in growth and macronutrients (N, P and K) at vegetative stage which directly enhanced the yield parameters of wheat plants. Moreover, these dual fertilizers caused significant increase in ions (N, P and K) and crude protein contents of grains. It can be concluded that the combination between biofertilizers and natural fertilizers are one of the best modern tools and can replace the chemical fertilizers.