POLLEN morphology and pattern of Nitraria retusa (Forssk.) Asch. were studied and compared against eight selected taxa of Zygophyllaceae viz. Fagonia arabica, F. cretica, Peganum harmala, Tribulus terrestis, Zygophyllum album, Z. coccenium, Z. decumbens and Z. simplexusing Scanning Electron Microscope (SEM) and sodium dodecyle sulphate-polyacrylamide gel electrophoresis technique (SDS-PAGE). A wide range of measurements were obtained from digitized SEM images of whole pollen grains and exine pattern. Twenty nine protein bands were obtained with 100% polymorphism among the species examined.F. arabica and T. terrestis were characterized by one positive unique band with a molecular weight 56.8 and 24 kDa respectively.P. harmula was characterized by two positive unique bands with a molecular weight 62 and 42 kDa. N. retusa was characterized by three positive unique bands with amolecular weigh 64.6, 60.7 and 36 kDa. A dendrogram was constructed based on the similarity data matrix by unweighted pair group method using arithmetic averages cluster analysis. SEM and protein pattern analysis recommended the separation of Nitraria retusa (Forssk.) Asch. besides Fagonia sp. into a distinct family.