RECENT progress in atmospheric plasma has led to creation of cold plasma with ions temperature close to room temperature. In present study the effects of cold atmospheric plasma jet on Ehrlich Carcinoma (EC) has been revealed. The present study is carried out on three groups of Males Swiss albino mice bearing Ehrlich Carcinoma. Design of suitable plasma source is used for the treatment. Tumors are ablated with a single transdermal cold atmospheric plasma (CAP) treatment. Tumor volume and mice survival parameters are determined. Results indicate that the mechanism of action for cold plasma on cancer cells is due to generation of reactive oxygen species (ROS). This is occurred with possible induction of the apoptosis pathways. The results of this study reveal that the CAP greatly inhibits Ehrlich Carcinoma cells (ECC) growth. This is considered as an advanced new modality for treatment of cancer cells with least side effects.