NANOTECHNOLOGY has emerged in the past decades with ……different disciplines including medicine that plays an important role in improvement of human health. Utilization of nanomaterials in antimicrobial application may help to avoid drawbacks of antibiotics or help to improve their antimicrobial effect against resistance of microorganisms. In the present work, silver (Ag) and zinc oxide (ZnO) nanoparticles (NPs) are synthesized and characterized using UV-Vis spectroscopy, transmission electron microscope (TEM), X-Ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR). The antimicrobial activities of the synthesized ZnO and Ag NPs are carried out by the well diffusion and a microtiter methods against different pathogens (Fungi, Gram-positive and Gram-negative bacteria) to reveal their minimum inhibitory concentration (MIC) and the half-maximal inhibitory concentration (IC50). The obtained results indicate that both types of nanoparticles are bacteriostatic and bactericidal while silver is more potent than ZnO NPs.