The clupeid, Nannothrissa stewarti (Poll and Roberts 1976), endemic to Lake Mai-Ndombe, is one of the most heavily fished fish species using practices and nets not allowed by the country's legislation. The objective of this study was to determine some aspects of the reproductive biology of N. stewarti in Lake Mai-Ndombe. Fish were monthly sampled from November 2020 to October 2021. Breeding parameters were determined: gonado-somatic index (GSI), size at first sexual maturity, absolute fecundity and the relationship between total weight (TW) and total length (TL). Results obtained showed that the sex ratio was in favor of females (1: 0.8). Estimated absolute fecundity was between 227 and 4080 oocytes for females of total length between 23 and 35mm, with an average of 923 ± 664g oocytes and a relative fecundity varying between 25120 and 155460 oocytes kg-1. The average oocyte diameter was 0.20 ± 0.14mm. Distribution of oocyte diameters observed in the population as well as monthly variations of the Somatic Gonado Index (SGI) indicates that the species has two main clutches during the year. LT₅₀ size at first sexual maturity is 27.6mm for males and 25.5mm for females. N. stewarti from Lake Mai-Ndombe has multiple reproductions throughout the year, with two maximum peaks at the beginning of the peak rainfall (February-March and September-October).