Drinking water sources suffer at the present time from great pollution as a result of population, industrial and agricultural growth and expansion, as well as the lack of proper planning in building cities. Since the Tigris River is the only water resource for the city of Mosul, the current study was conducted to assess the pollution of river water in the city of Mosul via estimating the concentration of some heavy metals (Fe, Co, pb, Zn and Cu) in the river water, passing through the city of Mosul. This work examined the water quality of the Tigris River to maintain its good quality and cleanliness. The sampling sites extend from the Kubba area in the North, where the river enters the city of Mosul to the Yarmajeh area in the South, where the river leaves the city for a period of 6 months, starting from September 2020 to February 2021. The results showed that the average water temperature ranged between 10 and 20°C. The values of the copper element were imperceptible during the study period, while the zinc element recorded concentrations ranging between 0.443 and 1.163mg/ liter in the river water, and the values of lead and cobalt ranged between 0.222- 3.770 and 0.750- 2.835mg/ liter, respectively; while, the iron element in the river water showed values ranging between 0.502 and 1.714mg/ liter. The results indicate that the heavy elements (lead, cobalt, and iron) exceeded the standard parameters allowed by the World Health Organization for the water of the Tigris River passing through the city, and the last location (the Yarmajeh area) was the recorded the most polluted site under study.