Mullets (Family: Mugilidae) are the most common fish species in Suez Canal lakes, contributing about 12% of the total lakes catch. They constitute a fundamental protein resource for the local communities living around the Suez Canal. The present study aimed to investigate the otolith microstructure and shape indices of Chelon ramada  form the Great Bitter and Timsah Lakes. The right and left otoliths were extracted from each fish individual after measuring to the total length and weighing. The otoith's length, width, area, perimeter in addition to the shape indices were described for specimens collected from each lake separately. Results revealed that there are significant differences in otolith shape and characteristics between the two fishing areas. Based on the obtained results, the otolith of  C. ramada could be used as a useful tool to differentiate between the different localities that have different ecological parameters.