The objective of the current research was to cultivate Micractinium reisseri (JN169781), a promising microalga for high biomass and lipid accumulation. The algal culture was exposed to 0.71 and 0.94 g L^-1 of sodium chloride (NaCl) for 20 days, resulting in 3.66 and 6.71 % increases in the growth, and biomass productivity (3.82 and 12.56 %), respectively at the two NaCl concentrations over the control. The growth and biomass productivity of M. reisseri gradually decreased with increasing seawater ratios (25, 50, 75, and 100%), however the highest lipid content and lipid productivity at 25 % SW concentration. M. reisseri was observed the highest biomass at 1 g L^-1 of glucose. The greatest levels of protein, lipid content, and lipid productivity were obtained at 5 g L^-1. With 1 g L^-1 Na-acetate, M. reisseri showed a high increase in growth and biomass productivity, while the greatest growth and biomass productivity were observed at 2 g L^-1 of NaHCO₃. In comparison to the control, the maximum protein content (32.37%) was found at 5 g L-1 of NaHCO₃, while the highest lipid productivity was found at 2 g L^-1 of NaHCO₃. The maximum growth, biomass, protein, lipid, and lipid productivity of M. reisseri were achieved at 0.1 g L^-1 glycerol. Cultivation of M. reisseri at 2 g L^-1 Na-acetate obtained the maximum protein, lipid, and lipid productivity. The results clarified that the culture of M. reisseri grown with enriched sodium salt and few carbon sources produced great biomass and lipid productivity