Information on the modern state of fish fauna in the Chirchik River (the largest tributary of the River Syrdarya, Uzbekistan) is very limited; hence the current study was organized to analyze the qualitative state of the ichthyofauna. Fish sampling was monthly conducted from February 2021 to March 2022 at the upper, middle, and down streams of the Chirchik River. The fish samples were taxonomically identified. The results revealed the addressed ichthyofauna included 39 fish species belonging to 12 families. Among them, 17 species are native, 22 species are invasive as a result of the acclimatization work in the second half of the 20th century.