Search for alternatives to animal models in biomedical and allied research is a recent trend in basic drug research communities; Zebrafish (Danio rerio) (ZF) would be an alternative animal model for biomedical research. Various animal model(s) have been used to study the cause and mechanism behind central nervous system disorder. However, still there is no clear evidence that triggers the cause of disease in these models. Currently, both larval and adult ZF emerged in scientific research as an alternative to in-vivo vertebrate species for CNS disorders due to their physiological and genetic similarity with humans. ZF research has characterized the various genes involved in neurodegenerative diseases and the same are found in rodents models. The use of ZF models is in line with the 3Rs principle of CPCSEA. In the present review, we summarize the ZF as an alternative to the animal model in CNS disorders and also highlight the advantage of ZF in basic biomedical research