256201

Characterization of amylase-producing Streptomyces sp. NAA-28 isolated from mangrove sediment, Red Sea, Egypt

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Last updated: 01 Jan 2025

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Abstract

Hydrolytic enzymes produced by actinomycetes are of outstanding quality and have important industrial uses. Streptomyces sp. NAA-28, an amylase-producing bacterium, was isolated from mangrove sediments and was chosen for comprehensive characterization and optimization of amylase production, based on its higher amylolytic activity as determined by the formation of a clear zone using starch agar medium. The strain was identified based on the biochemical and morphological characteristics and the sequence of the 16s rDNA gene. The sequence was deposited in GenBank under the accession number MZ496703. The optimum NaCl concentration for amylase production was 6%. The strain produced the highest amount of amylase after 5 days of incubation at 35°C in the culture medium adjusted to pH 8 and supplemented with peptone and starch as carbon and nitrogen sources, respectively. Further studies are required to purify and characterize the secreted enzyme to determine its potential for industrial applications.

DOI

10.21608/ejabf.2022.256201

Keywords

Streptomyces, Mangrove, Red Sea, amylase, Optimization

Authors

First Name

Nayer M.

Last Name

Fahmy

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Volume

26

Article Issue

4

Related Issue

35549

Issue Date

2022-07-01

Receive Date

2022-08-25

Publish Date

2022-07-01

Page Start

1,053

Page End

1,065

Print ISSN

1110-6131

Online ISSN

2536-9814

Link

https://ejabf.journals.ekb.eg/article_256201.html

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https://ejabf.journals.ekb.eg/service?article_code=256201

Order

62

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Original Article

Type Code

103

Publication Type

Journal

Publication Title

Egyptian Journal of Aquatic Biology and Fisheries

Publication Link

https://ejabf.journals.ekb.eg/

MainTitle

Characterization of amylase-producing Streptomyces sp. NAA-28 isolated from mangrove sediment, Red Sea, Egypt

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Article

Created At

22 Jan 2023