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255472

Mutagenic effect of the sex reversal hormone Diethylstilbestrol in the Nile tilapia (Oreochromis niloticus, L) through sister chromatid exchange analysis

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Last updated: 01 Jan 2025

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Abstract

The current study was conducted to explore the mutagenic effect of DES hormone on the treated Nile tilapia chromosomes using sister chromatid exchange (SCE) analysis. Two concentrations of DES hormone (100mg DES/kg and 200mg DES/kg diet) were addressed. The fry were fed for 60 days at 15% of the body weight daily. Two cell replication cycles, in the presence of 5-bromodeoxyuridine (BrdU) were achieved, and intestine tissues were used for chromosomes preparation. For slide staining, acridine orange (AO) was added, and the fluorescent microscope was used to the observation of AO fluorescence of stained slides. The obtained results indicated that the frequency of SCEs per cell and the percentage of cells exhibiting three SCEs per cell increased with the increasing hormone concentration. The present findings confirmed the mutagenic effect of DES hormone and clarified the possibility of using O. niloticus as an in vivo system for detecting mutagenic and carcinogenic chemicals. In addition, it is recommended not to use the hormone directly in the food served to humans. However, it can be used in the induction of fish sex inversion to XY females and followed by progeny testing, to generate super male YY.

DOI

10.21608/ejabf.2022.255472

Keywords

DES, Oreochromis niloticus, SCEs analysis, sister chromatid exchange

Authors

First Name

Medhat H.

Last Name

Hashem

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Orcid

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Volume

26

Article Issue

4

Related Issue

35549

Issue Date

2022-07-01

Receive Date

2022-08-19

Publish Date

2022-07-01

Page Start

875

Page End

883

Print ISSN

1110-6131

Online ISSN

2536-9814

Article File

EJABF_Volume 26_Issue 4_Pages 875-883.pdf

PDF . 360.0kB

Link

https://ejabf.journals.ekb.eg/article_255472.html

Detail API

https://ejabf.journals.ekb.eg/service?article_code=255472

Order

51

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Original Article

Type Code

103

Publication Type

Journal

Publication Title

Egyptian Journal of Aquatic Biology and Fisheries

Publication Link

https://ejabf.journals.ekb.eg/

MainTitle

Mutagenic effect of the sex reversal hormone Diethylstilbestrol in the Nile tilapia (Oreochromis niloticus, L) through sister chromatid exchange analysis

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Article

Created At

22 Jan 2023