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249977

Assessment of the antioxidant capacity of Lanistes carinatus tissue extract and its immune-boosting influence on Biomphalaria alexandrina against infection with Schistosoma mansoni

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Last updated: 22 Jan 2023

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Abstract

Schistosomiasis remains a public health problem in many developing countries including Egypt. The disease control initiatives stress on control of snail intermediate hosts side by side with the treatment of infected human cases. Blocking the parasite transformation within the snails will interrupt schistosomiasis transmission. Thus, the present study assessed the antioxidant efficacy of Lanistes carinatus tissue extracts and its role as an immunostimulant for Biomphalaria alexandrina against infection with Schistosoma mansoni. L. carinatus soft tissues were extracted with methanol and the resulting crude extract was further fractioned with petroleum ether, methylene chloride, and ethyl acetate (EtOAc). The total antioxidant capacity (TAC) and total phenolic content (TPC) of all extracts were determined. Furthermore, the effect of the highest antioxidant extract on hemocytes activity (phagocytosis), antioxidant parameters, and infection rate of B. alexandrina was assessed. The results showed that EtOAc extract had the highest antioxidant capacity and total phenolic contents. EtOAc extract led to activation of the phagocytic process with a significant increase in the phagocytic indexes of hemocytes after 1, 2, and 4 h of exposure. In addition, it increased the levels of total antioxidant capacity, superoxide dismutase, reduced glutathione, and glutathione-s-transferase in B. alexandrina compared to controls. The infection rate of snails was significantly reduced due to EtOAc extract exposure. These results collectively suggest that EtOAc extract from L. carinatus has an antioxidant capacity and immuostimulatory effect for B. alexandrina infectied with S. mansoni

DOI

10.21608/ejabf.2022.249977

Keywords

Lanistes carinatus, extracts, Biomphalaria alexandrina, Schistosoma mansoni, Infection

Authors

First Name

Rasha M.

Last Name

Gad El-Karim et al.

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Volume

26

Article Issue

4

Related Issue

35549

Issue Date

2022-07-01

Receive Date

2022-07-20

Publish Date

2022-07-01

Page Start

361

Page End

376

Print ISSN

1110-6131

Online ISSN

2536-9814

Link

https://ejabf.journals.ekb.eg/article_249977.html

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https://ejabf.journals.ekb.eg/service?article_code=249977

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22

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Original Article

Type Code

103

Publication Type

Journal

Publication Title

Egyptian Journal of Aquatic Biology and Fisheries

Publication Link

https://ejabf.journals.ekb.eg/

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Article

Created At

22 Jan 2023