Saline groundwater is the substitute for seawater; especially when it is not possible to establish the hatcheries near the shore. The aim of this study is to assess the suitability of untreated or treated saline groundwater for the survival, growth and reproduction of L. ramada broodstock. The treatment of saline underground water was carried out in three steps: mechanical filtration, chemical treatment with chlorine and biological treatment with green algae; filamentous algae and Nannochloropsis occulata. Immature mullet broodstock was stocked in different waters; treated and untreated saline groundwater, for six months to follow up on growth performance and reproductive activity in these waters. High levels of some elements such as iron and manganese as well as hydrogen sulfide gas and high levels of ammonia were observed in the untreated saline groundwater in comparison to those of treated water. The first step of water treatment is the mechanical stage, where the water is filtered mechanically, in which hydrogen sulfide gas is disposed of, then the second chemical stage uses chlorine, which oxidized and precipitated both iron and manganese, followed by the third biological stage, in which green algae; filamentous algae and N. occulata are used, which work to eliminate the ammonia. The treatment of saline groundwater increased the survival rate and improved the growth of mullet broodstock; since high values of growth in length and weight and better condition factors were recorded for fish stocked in treated water. In addition, the reproductive activity of mullet broodstock was increased in treated water. High values of hepatosomatic index (HSI), gonadosomatic index (GSI), and a high percentage of frequency for maturity gonad stages were obtained in treated saline groundwater. The present study concluded that treatment of saline groundwater is essential for the improvement of water quality, growth performance, and the reproductive activity of mullet broodstock.