Nowadays there is a general demand for green chemistry to keep our environment. Chlorophyllin is widely used as a promising natural pesticide instead of a chemical one, which raises the concern for studying its eco-toxicological effect on the surrounding environment. In this study, both silver and graphene oxide nano-materials were grafted by porphyrin-based chlorophyllin to produce four novel natural nano-pesticides (Mg-Chl/Ag, Mg-Chl/GO, Cu-Chl/Ag, and Cu-Chl/GO), their effects on the physicochemical properties of water and Clarias gariepinus (C. gariepinus) health were evaluated. Each nano-composite was used in three dilutions; 102, 10-3, and 10-4 ml/l. The obtained results indicated that such nano-composites maintain the ionic composition of the surrounding water within the international permissible limits, while high concentrations caused increased BOD, COD, NH4, and NO3 values. Using such nano-composites at High concentrations also alters the hematological and biochemical functions of C. gariepinus; they caused a decrease in RBCs count, Hb, Hct %, serum protein, and globulin concentrations. Also, there was an increase in total WBCs count, serum albumin, ALT, AST, glucose, cholesterol, creatinine, and uric acid concentrations. This study provides the environmental impact of some novel natural nano-pesticides and points to their possible toxic effects on fish health, especially at higher concentrations.