Philophthalmus species (the eye flukes) are digenetic trematodes that cause damage to the ocular tissues of birds and humans. Diagnosis of subclinical philophthalmosis and effective therapeutic regimens are lacking, despite the increasing number of cases reported in birds and humans. So, the present study aimed to evaluate in vitro the antihelminthic activity of Praziquantel (PZQ), Flubendazole, and plant leaves extracts (Jatropha curcas and Moringa oleifera) against the adult eye fluke Philophthalamus palpebrarum(Philophthalmidae) which extracted from the conjunctival sac of the experimentally infected chickens eyes by excysted metacercariae The cercariae obtained from the naturally infected freshwater snails Melanoides tuberculata collected from watercourses in  Giza Governorate, Egypt. The efficacy was determined based on the dead worms percentage after treatment and morphological changes recorded by scanning electron microscopy. The worms were incubated in the culture media with different concentrations tested materials up to 72 h. The calculated LC₅₀were 7.857, 9.170, 65.534 &44.509μg/mL and LC₉₀were 10.149, 13.489, 91.474 & 53.004μg/mL, respectively. Worms incubated for 48h with LC₅₀ of PZQ and Moringa revealed deformity, swelling of oral and ventral suckers, erosion, and disorganization of the tegument when examined with scanning electron microscopy. It can be concluded that Praziquantel, Flubendazole, Jatropha curcas, and Moringa oleifera plant leaves extracts possess in vitro antiphilophthalamus activities; however, studies in vivo should be conducted to examine their antiphilophthalamus effects.