In the River Nile, water pollution is a fundamentally environmental and public health issue that requires deep consideration. Heavy metals (copper "Cu", zinc "Zn", iron "Fe", cadmium "Cd", manganese "Mn", chromium "Cr", and cobalt "Co") were found in the sediment, water, and three aquatic insects (Crocothemis sp., Diplonychus urinator, and Micronecta isis) at two sites along Al-Mahmoudia canal during a study extending from summer 2019 to spring 2020. In water and sediment, Fe, Mn, and Zn concentrations recorded their highest values, followed by Cu and Cd, whereas Cr concentrations were lower in both seasons. In addition, the highest accumulation seasons were summer and spring, while the lowest accumulation season was winter. In aquatic insects, the highest amounts of Fe, Zn, and Mn were detected, followed by Cu, Cd, Co, and Cr. The lowest bioaccumulation factor (BAF) was recorded for Co, Mn, and Cu, while Fe and Zn showed the greatest. Additionally, the metal pollution index (MPI) value was high in summer and low during winter. In this context, Fe, Mn, and Cu in water and sediment reflected their utmost metal effect on the investigated insects. Furthermore, Mn and Co were more accumulated inside Crocothemis sp., while Cu and Cd were more accumulated inside Diplonychus urinator, but Zn showed the same accumulation rate inside all insects.  [m1]identify the 2 seasons.