Identification of freshwater fish's bacterial flora is an important tool for the determination of quality and the safety of human consumption. The aim of the present study was to isolate the bacterial flora of freshwater fish from gills, buccal cavity and skin surface of Schizothorax esocinus, from the locations of river Jhelum of Kashmir valley. A total number of 45 samples of freshwater fish were selected on the basis of clinical diagnosis. The fish sample was distributed on the basis of clinical inspection as gills [20%, (n=9)], skin [11.11%, (n=5)], gills and skin [24.44%, (n=11)], gills and buccal cavity [13.33%, (n=6)], skin and buccal cavity [8.90%, (n=4)], gills, skin and buccal cavity [22.22%, (n=10)], respectively. Statistically, the frequency distribution was not uniform and p < 0.5. Freshwater fish's bacterial flora consists of Pseudomonas aeruginosa, Staphylococcus sp., Enterococcus sp., Bacillus sp., Salmonella sp < em>. and Escherichia coli. The isolation of bacterial flora in fish serves as an indicator organism of anthropogenic pressures/activities of the aquatic environment. Their presence causes diseases for humans. The association of pathogenic bacterial species in fish may lead to food safety hazards for the consumers if the fish are handled or prepared improperly.