Two experimental trawl surveys were conducted in the Gulf of Suez during[m1]  April (spring) and November (autumn) 2014. The current study addressed the effect of the diurnal variables (day and night) on the catch rates of three fish species (lizard fish Saurida undosquamis, horse mackerel Trachurus indicus, threadfin bream Nemipterus japonicus), two cephalopods (cuttlefish Sepia sp, Indian squid Loligo duvauceli) and two crustaceans (Crab Portunus pelagicus and shrimp < em> Penaeus sp.). In both surveys, the highest catch was caught just after dusk during the daytime, while during the night, it was recorded in the middle of the night. In spring, day and night mean catch rates were not significantly different (P˃0.01) for all species. While in autumn, the rates of Trachurus indicus, Loligo duvauceli, and Penaeus sp. were significantly different (P ˂ 0.01 and T values were 2.80, 3.28 and 2.34, respectively). In addition, those of Saurida undosquamis, Nemipterus japonicus, Sepia sp., and Portunus pelagicus were not significantly different (P˃0.01). The results of the study revealed that both the early morning and the late evening are the best times for fishing.  [m1]