Hypophyseal homogenate of common carp is considered one of the different hormonal sources for spawning induction in the teleosts. The present work aimed to measure the hormonal concentration of the hypophysis in different carp species, which can be used as a source of hormones for spawning induction in teleosts. The hypophysis of different mature carp species (both males and females); namely, common carp (Cyprinus carpio), grass carp (Ctenopharyngodon idella) and silver carp (Hypophthalmichthys molitrix) were evaluated biochemically to discuss their hormonal source. Five hormones in the pituitary gland, including adrenocorticotropin hormone (ACTH), prolactin hormone (PRL), growth hormone (GH), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were analyzed using electrochemiluminescence immunoassay (ECLIA). Compared to female carps, the hypophysis homogenate of male carps recorded lower levels of the measured hormones. The biochemical results showed that the hypophysis homogenate of H. molitrix showed higher levels of 426±13, 118±6, 2355±47, and 1005±35 ng/mg for PRL, GH, LH, and FSH, respectively. Whereas, lower level of ACTH (5±0.5 ng/mg) was recorded compared to those obtained in C. carpio and C. idella. However, the concentrations of those hormones recorded the lowest values in male carp fish, while the concentration of ACTH hormone was higher in male common carp, recording 13±1.2 ng/mg. The present biochemical observations revealed the suitability and effectiveness of the cheap silver carp hypophysis as a spawning inducer in fishes.