During the period extending from spring 2014 till winter 2015, macrobenthic invertebrates were collected from El Rayah El Tawfiky using two sampling methods; Ekman grab and macrophyte sweep netting. To address the composition, distribution and abundance of the species under study, thirty nine species were recorded, representing the sampling methods (20,505 and 3557 individuals/m²respectively).  Macrobenthic invertebrates collected belong to three phyla; namely, Arthropoda, Annelida and Mollusca.  Significant differences were detected in community composition, distribution and abundance of macrobenthos. Those differences were attributed to the varieties of the sampling methods, the seasons and the sites as well. In samples collected by a grap, the dominance of Annelida species was observed,  while the Arthropoda species prevailed in samples collected by macrophytes. The highest density was detected at stations VI and V in a grap and macrophyte sampling, respectively. Adversely, its lowest was determined at station VII with respect to both sampling methods. For the grap sampling, spring was the best season, whereas summer was the most preferred for macrophyte sampling. Results showed that the study area suffers from several sources of pollution, and the tolerant species such as Chironomidae larva and Limnodrilus udekemianus were the most dominant. Hence, a regular monitoring program is recommended to allow the detection of any future changes.