A hydrobiological investigation of macrobenthic fauna was seasonally conducted to address El-Rayah El-Nasery and El-Rayah El-Behery from spring 2014 to winter 2015. A total of 52 species were recorded belonging to three phyla. and represented by 33,438 and 12,066 individuals/m² in both rayahs, respectively. Arthropoda was the most predominant (30 species), followed by Mollusca (15 species) and Annelida  (7 species). Station- N8 recorded the highest density and diversity, while the lowest abundance and species number of macrobenthic fauna were found in station- B6. The latter station was proved to be contaminated with organic waste originated from ElRahawy drain. The diversity and density of macrobenthic fauna were higher in El-Rayah El-Nassery compared to El-Rayah El-Behery due to organic pollution in the latter flowing from the Rosetta Branch after the confluence with El Mahmoudia canal.