The Red Sea tiger snake eel Myrichthys maculosus belongs to the family Ophichthidae, order Anguilliformes, represented with 11 relative species and two outgroups for phylogenetic analysis by mean of large subunit 16S rRNA sequence. M. maculosus contains a high concentration of AT (53.8%) more than GC (46.1%), higher also in other understudying Ophichthidae species. The genetic distance values among M. maculosus and other related species ranged from 0.0006 to 0.029, while among all species ranged from 0.006 to 0.037%. Overall the distance value was 0.167%. The smallest genetic distance (0.006) was between M. maculosus (MW435681) and the same one under the accession number of DQ645692, while the largest distance (0.036%) was between Scolecenchelys breviceps and Ophichthus zophochir&O. apicalis. The three phylogenetic trees Maximum likelihood (ML), Neighbour Joining (NJ), and Minimum Evolution (ME) applied here showed the same relations with slightly different in support values.