The Egyptian government seeks to develop the northern wetlands, including Edku Lake in El Beheira Governorate, to enhance the physiological functions of fish, and thus, improve their quality and promote their products. Therefore, this study was performed on the two cheapest fish species Tilapia zillii and Clarias gariepinus in the Egyptian market, by studying proximate body composition, antioxidant enzyme activity, and bioaccumulation of five trace elements. In this study, T. zillii showed the highest mean of carbohydrates (16.11± 0.67 mg/g), fats (24.21 ± 0.93mg/g), ash (7.33 ±1.00 %), and water content (78.33 ± 1.85 %) whereas, the highest mean of protein content was recorded in C. gariepinus (64.33 ±1.01 mg/g). Antioxidants glutathione reduced level (GSH) (41.66 ± 2. 01 mg/g) and catalase activity (CAT) (21.66 ± 2.6 U/g) demonstrated a significant decrease (p < /em> < 0.05) in the liver of C.gariepinus than T. zillii (47.83 ± 3.6 mg/g and 38.16 ± 4.2 U/g, respectively). Concerning trace element concentrations, the highest arsenic (As) concentration was recorded in tissues of C. gariepinus (1.54 ± 0.08 μg/g), whereas, the lowest mercury (Hg) concentration was recorded in the tissues of T.zillii (0.47 ± 0.08 μg/g). The concentrations of mercury (Hg), cadmium (Cd), and lead (Pb) in the two studied fish species exceeded the limits permitted by EOSQC,  FAO/WHO, and the EU'. This study recorded that the children should consume less than 16.12857 g/day or 112.90 g/week of T. zillii muscle and 13.27 g/day or 92.92 g/week of  C. gariepinus muscle. Moreover, youth should consume less than 43.01 g/day or 301.7g/ week of T. zillii muscle and 35.39714 g/day or 247.78 g/week of C. gariepinus muscle. Finally, adults should consume less than 23.1 g/day and 161.7 g/week of T.zillii muscle, and 61.94286 g/day or 433.6 g/week of C. gariepinus muscle to maintain their safety based on PTWI recommended by FAO/WHO.