Macrobenthos are ecological indicators and also work as biological engineers in any aquatic ecosystem. The present study aims to assess the abundance and distribution of macrobenthos along the Gomti river, Bangladesh during the post-monsoon season. A total of 11 families of macrobenthos were identified during the study period and reporting to be 58400 ind./m2 (mean 7300 ind./m2) from the 8 sampling stations. The maximum abundance (15200 ind./m2) was found at station 2 and the minimum (1300 ind./m2) at station 3. The variations in the macrobenthos community were identified, and the species composition of the community showed that Oligochaeta was the dominant group (83.05%) followed by Insecta (10.27%), Gastropoda (2.57%), Gymnolaemata (2.23%), Polychaeta (1.54%) and Malacostraca (0.34%). The alpha diversity (taxa distribution) was the highest (α= 6) at station 4 and the lowest (α= 3) at stations 1 and 3. Moreover, the values of different diversity indices; Simpson diversity index (0.52-0.66), Shannon-Wiener index (0.85-1.25), Evenness index (0.71-0.99), Margalef Species Richness Index (0.2-0.51), and Equitability index (0.78-0.99) indicated an intermediate state of environmental stress and pollution. The information gathered here could be useful to measure the impact of pollution and to conserve these riverine habitats' biodiversity.