Hybrid strains of the genus Pleurotus (PO₅XPD₄ and PO₉XPD₅) were grown in glucose medium and yeast extract in an air transport bioreactor (using different pHs 3.5, 4.5, 5.5, 6.5, and 7.5) for the production of mycelial biomass and exopolysaccharides (EPS). The highest biomass content (13.15 g L^-1) and the highest production of exopolysaccharides (1.95 g L^-1) were presented in the propagation of PO₅XPD₄ at pH 3.5. The liquid cultures under pH of 3.5 used in the cultivation of hybrid strains presented the highest production of exopolysaccharides that can be used to remove aquaculture effluents. The results showed that the production of biomass and exopolysaccharides (EPS) is directly related to the pH and the strain.